• High Grade Aggressive B Cell Lymphomas (Double & Triple-Hit Lymphomas)

• The WHO Classification in 2017 classified lymphomas with concurrent rearrangement of MYC, BCL2, and/or BCL6 as high-grade B cell lymphomas with an aggressive clinical course.

• Double-hit and triple-hit lymphomas are difficult to classify morphologically without aid of FISH.

• The diagnosis is based on interphase FISH for MYC, BCL2, and BCL6.

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Sample Requirements:

• Peripheral Blood or Bone Marrow

  • 2-5mL in a sodium heparin (green top) tube. 5 mL in a EDTA (purple top) will be accepted but is not preferred.

  • Minimum: 1 mL

• Storage: Room temperature if being shipped within 24 hours.  Refrigerate if shipping will be delayed.

• Stability: Sample is optimum if received within 48 hours

• Transportation: Room temperature (do not freeze or fix)

• FFPE Tissue sections

  • 4-micron sections on positively charged slides; include one H&E slide

  • Minimum= 2 slides

• Storage: Room temperature

• Stability: Indefinite if stored at room temperature or refrigerated

• Transportation: Room temperature. In summer, add cold pack to prevent melting

• Lymph Node tissue

  • Fresh tissue completely submerged in sterile culture media (such as RPMl)

  • Multiple containers accepted

• Storage: Room temperature if being shipped within 24 hours. Refrigerate if shipping will be delayed.

• Stability: Sample is optimum if received within 48 hours

• Transportation: Room temperature (do not freeze or fix)